Background: Osteomyelitis (OM) is a risk factor for hospitalization, lower extremity amputation and impaired DFU wound healing. The aims of the present study were 1) to describe the clinical characteristics of individuals with diabetes and OM and 2) to investigate the relationship between the healing rate and DFU-related parameters including the type of involved pathogens (Gram+ vs Gram-). Methods: One hundred and forty-two individuals with type 2 diabetes (T2DM) were treated for OM in a tertiary-care center and had a regular follow-up for one year. Laboratory measurements and clinical parameters, including DFU- and OM-related assessments were gathered from medical records. People included were divided in two groups according to the ulcer outcome [Healed (H) group, n=84, Not Healed (NH) group, n=58]. Results: Compared with H group, NH group included significant lower proportion of women (H vs NH, 38% vs 21%, p=0.043). There were significant differences in levels of creatinine [median (IQR), 0.8 (0.7,1.2) vs 1.2 (0.9,1.6), p<0.001] and albuminuria [26.7 (17.2,62.2) vs 108.5 (24.0,500), p=0.003], estimated glomerular filtration rate (eGFR) [80.3 (61.0,102.0) vs 59.0 (41.4,85.6), p=0.001] favoring people in H group. Gram+ infections were more frequent in NH group, as compared to H group (52% vs 72%, p=0.026), with a healing rate reduction of 58% (Relative Risk 0.42, 95%CI 0.20-0.86, p<0.02). No differences were found between groups in other parameters. The multiple logistic regression analysis identified male sex, low eGFR and Gram+ infection as predictors of healing failure. Conclusions: In a population of people with T2DM and OM treated in a tertiary-care center, male sex, reduced eGFR and Gram+ infection were related to poor healing within one year follow-up.